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Title: Interaction of Herpes Simplex Virus Type-1 with the host cell surface
Author: Hawkins, C.
Awarding Body: University of Cambridge
Current Institution: University of Cambridge
Date of Award: 2001
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The role of GAGs in HSV-1 SC16 adsorption and entry, was examined here in the context of three different cell types using infection as a readout. These experiments demonstrate that the importance of GAGs in infection varies from one cell type to another, reinforcing the view that a role for GAGs as entry receptors is not universal for all virus - cell type combinations. As well as participating in receptor-mediated attachment and playing a crucial role in virion envelope-plasma membrane fusion, HSV-1 gD is also essential for cell-associated spread. On the basis that some of the essential functions of the gD of other related herpes viruses can become dispensable under certain conditions, a study was carried out to determine whether a variant of HSV-1, capable of gD-independent cell-associated spread could be isolated. However, these attempts were unsuccessful in the context of HSV-1, implying that the function of gD in cell-associated spread cannot be compensated for in other parts of the virus. Finally, the issue of secretion and release of HSV-1 progeny virus particles was examined. Electron microscopy studies established that large numbers of virus particles are present on the infected cell surface, and that the bottle-neck to production of virus in the tissue culture medium is release from the infected cell surface rather than secretion through the plasma membrane. Since it is known that components of virus particles interact with GAGs, the role of GAGs in release of progeny virions from the cell surface was investigated. In this way it was confirmed that in some cell types, the presence of GAGs is important in retaining progeny virus in a cell-associated state.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available