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Title: The colonic and vascular health effects of coffee : the effects of processing
Author: Mills, Charlotte E.
Awarding Body: University of Reading
Current Institution: University of Reading
Date of Award: 2013
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There is increasing evidence suggesting that polyphenol rich diets are associated with decreases in degenerative diseases such as cardiovascular and neurodegenerative diseases and some cancers. Coffee is abundant in such compounds called chlorogenic acids (CGA) but has gained limited attention with regards to health effects. As an extremely popular beverage internationally, coffee is likely to be a valuable source of dietary polyphenols. In this thesis we show that there is wide variation in the levels of CGA in a selection of commercial coffee (27.33- 121.25 mg/200 mL coffee brew) which seems to be driven predominantly by the roasting process. This highlights the importance of coffee choice to achieve an optimum intake of CGA. Further, we explore how consumption of commercial coffee may impact gut health. Through in vitro batch culture fermentation we show that coffee and CGA possess the ability to modulate the gut microbiota through selective metabolism. Notable significant increases were observed with Bifidobacterium spp. relative to the control at 10 hours post inoculation of both CGA (80.8 mg 5-caffeoylquinic acid) and the high CGA coffee (80.8 mg CGA). Finally, we also assessed the effect of high and low CGA coffee (236 mg and 108 mg total CGA respectively) on vascular health, measured as endothelial function. In this human intervention study, we observed a biphasic change in flow mediated dilatation (FMD) in response to both coffees I and 5 hours after consumption but not to the caffeine control (0 mg CGA). This response in endothelial function correlated with total plasma CGA metabolites (r= 0.21 , p= 0.005), concluding that circulating CGA metabolites might be responsible for the vascular effect. Overall, our results provide evidence that, like other polyphenols, CGA delivered in coffee could potentially have positive effects on the gut microbiota and vascular system.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available