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Title: Bioactive peptides from the skin secretion of the Central American red-eyed leaf frog, Agalychnis callidryas
Author: Li , Renjie
Awarding Body: Queen's University Belfast
Current Institution: Queen's University Belfast
Date of Award: 2013
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Frogs belong to the amphibian Order, Anura, and are distributed around the wor1d from the tropics to sub-Arctic regions. Over long periods of time, frogs have generated an astonishing range of adaptations to suit their discrete environments and to survive intense biological competition. Among these adaptations, the skin has been central as one of the paramount organs with broad functions such as absorption of water and oxygen, camouflage and resisting predation, to mention a few. Studies on the myriad chemical compounds that are present in various frog skins, has revealed their potential as a novel source of natural drugs. The technologies employed in this thesis were focused on the discovery of novel bioactive peptides from the skin secretion of the Central American red-eyed leaf frog, Agalychnis callidryas. Peptidomic studies were initiated with biological activity screening which helped us to locate chromatographic fractions that contained functional peptides, in the first instance. Then MS/MS fragmentation sequencing or Adman degradation was employed to identify the accurate amino acid sequences of the active peptides. Genomic studies were then initiated using molecular cloning to isolate the skin-derived canvas encoding the precursors of the novel peptides identified. The comprehensive approach using these methods in parallel, resulted in the identification of 11 bioactive peptides whose functions ranged from antimicrobial activity, tryps in inhibition activity, myotropic activity and anticancer activity. This thesis consists of 9 chapters that introduce the background information relating to the project, the methods used in the research, the experimental data and finally, a general discussion of the relevance of the findings. The experimental chapters, 3 through 8, describe novel peptides from this species of frog and new strategies for amphibian skin peptide discovery.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available