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Title: Whole blood platelet function testing as an adjunct to thrombophilia screening
Author: Wakeman, Lisa Jayne
Awarding Body: University of the West of England, Bristol
Current Institution: University of the West of England, Bristol
Date of Award: 2013
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Platelet hyperaggregability, including Sticky Platelet Syndrome has been identified as a cause of thrombotic conditions including venous thrombosis and foetal loss. The laboratory diagnosis of classical Sticky Platelet Syndrome is based on enhanced platelet aggregation responses to submaximallevels of adenosine diphosphate and epinephrine by optical aggregometry techniques. This study sought to evaluate platelet hyperaggregability by Multiple Electrode Aggregometry, PFA-100 analysis and quantitative analysis of associated platelet parameters as an adjunct to thrombophilia screening. Forty nine patients and 50 control subjects (nonna! volunteers) were investigated in this study. Multiple Electrode Aggregometry was performed on all participants at standard and submaxima! concentrations of adenosine diphosphate, collagen, thrombin receptor agonist protein-6, arachidonic acid and epinephrine together with PFA-100 closure times, immature platelet fraction, mean platelet volume, plateletcrit, platelet distribution width and platelet-large cell ratio measurement. Statistically significant differences in PFA-100 (collagen/adenosine diphosphate cartridges) were observed between the test and control cohort. Observations of study results and clinical presentation in the test cohort identified results outside of reference ranges in symptomatic and asymptomatic test individuals in whom no abnormalities were recorded by conventional thrombophilia testing. Binary logistic regression models identified that mean platelet volume levels were found to contribute significantly to the prediction of whether an individual presented as a member of the test or control cohort. Mean platelet volume was also shown to contribute significantly to predicting whether a participant feU into test and control subgroups. Study results suggest that evaluation of platelet reactivity by whole blood platelet analysis may play a role as an adjunct to conventional laboratory investigation of patients who present for thrombophilia screening.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available