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Title: The study of techniques for impedimetric discrimination of toxins using a whole cell biosensor
Author: Djawad , Vasser Abdul
Awarding Body: University of the West of England, Bristol
Current Institution: University of the West of England, Bristol
Date of Award: 2013
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An impedance measurement system can be used to monitor the impedance changes of cells during a toxin challenge. The changes are associated with morphological transformations, organelle movement and metabolic activities. Currently, the measurement systems described in the literature involve non reusable and complex biosensors, as well as expensive instruments to study cell characteristics. An improved impedance measurement system is required to facilitate multiple sequential measurement using a reusable biosensor together with an inexpensive instrument. In addition, a new feature extraction technique is needed which can effectively identify different types of toxin and the concentration level applied to the cells. The purpose of this study is to design and analyse an improved impedance measurement system incorporating a whole cell biosensor and to develop a new feature extraction technique to discriminate the concentrations and types of toxin. This thesis presents the design, fabrication and evaluation of an impedance measurement system based on a lock-in amplifier to monitor the changes of adherent cells. Tests are described and discussed, and compared with a cell model of adherent cells. A program of cytotoxicity tests was conducted using this system to monitor the morphological cell changes and the output signals were analysed. Linear time-frequency representation techniques were used as an alternative to current methods to analyse the non-stationary signals. These techniques transform signals in the time domain into the timefrequency domain to provide more information about when a specific signal occured.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available