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Title: In vitro toxicity of new engineered nanoparticles through their life cycle
Author: Irfan, Adeel
Awarding Body: Cranfield University
Current Institution: Cranfield University
Date of Award: 2013
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The rapid development of nanotechnology has caused concerns about nanoproducts on human health throughout their lifecycle. As part of the consortium NEPHH (nanomaterial related environmental pollution on human health through their life cycle, funded by EU-FP7), this project aimed to assess the potential effect of novel polymer-silicon composites on human health from a lifecycle perspective, focusing on in vitro toxicity of raw silica nanoparticles (SiNP) and dust nanoparticles (NP) released from silicon-based polymer composites. The main objectives were to characterise a group of amorphous SiNP and dust NP in water and cell culture medium; assess NP toxicity potential in in vitro models; and establish mode of SiNP action. The selection of SiNP of size 7-14 nm was based on their wide use in developing polymer nanocomposites. Dust NP were generated from mechanical processing of polymer composites made of polyamide-6 (PA6), polyurethane (PU) and polypropylene (PP), each incorporated with SiNP or 3 other different silicon reinforcement materials. The dispersion and size of NP in water and in cell culture medium were characterized using dynamic light scattering, scanning electron microscopy and transmission electron microscopy. The chemical composition of NP was assessed by infra-red spectroscopy. NP were assessed in vitro for induction of membrane damage, intracellular reactive oxygen species (ROS), loss of cell viability, and cellular uptake by flow cytometry and confocal microscopy. In order to identify potential biomarkers for toxicity prediction, miRNA array and extracellular metabonomic assays were performed. Cont/d.
Supervisor: Zhu, Huijun; Njuguna, James Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available
Keywords: Nanotoxicology ; Silica ; in vitro ; physical characterisation ; life cycle analysis