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Title: Muscle differentiation in Drosophila : analysis of the roles of DMEF2 and Dmeso47C
Author: Gunthorpe, D.
Awarding Body: University of Cambridge
Current Institution: University of Cambridge
Date of Award: 2000
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In this dissertation, two approaches are taken to gain a greater understanding of muscle differentiation in Drosophila. The first is to analyse the role of a gene, Dmef2, which is already known, from work in both vertebrates and Drosophila, to be essential for this process. Two specific aspects of the role of Dmef2 in muscle differentiation are analysed: the function of different levels of the protein and the role of the different isoforms. It is shown that different properties within a cell require distinct threshold levels of DMEF2, as do different cells within a muscle-type and different muscle-types. Furthermore, there is a DMEF2 activity range that is compatible with the proper differentiation of different muscle-types. In addition, each DMEF2 isoform is found to function similarly, both when ectopically expressed in the ectoderm and, strikingly, in the rescue of each aspect of the Dmef2 mutant phenotype assayed. The second approach taken is to characterise and analyse the function of a novel gene, Dmeso47C, whose expression pattern in the somatic and visceral musculature is suggestive of a role in the differentiation of these muscle types. The full length coding sequence of Dmeso47C is obtained and shown to encode a putative nuclear protein. Both loss-of-function and gain-of-function experiments indicate that Dmeso47C does indeed play a role in muscle differentiation. Further, analysis of its expression pattern in different genetic backgrounds allows Dmeso47C to be placed in the genetic framework of visceral and somatic muscle differentiation.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available