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Title: Identification of genes interacting with Armadillo, the Drosophila homologue of beta-catenin
Author: Greaves, S.
Awarding Body: University of Cambridge
Current Institution: University of Cambridge
Date of Award: 1999
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Drosophila Armadillo is a multi functional protein implicated in both cell adhesion, as a catenin, and cell signalling, as part of the Wingless signal transduction pathway. Viable fly stocks have been generated which overexpress or underexpress Armadillo and as a result have distinctive phenotypes. Excess cytoplasmic Armadillo leads to an increase in vein material and bristles, whereas a reduction in cytoplasmic Armadillo yields a wing notching phenotype typical of weak wingless mutations. Both phenotypes can be dominantly modified by removing one copy of genes known to encode members of the Wingless pathway. Here, I will describe a genetic screen to identify further genes which dominantly modify the Armadillo mis-expression phenotypes. These genes represent three functional groups: establishment and maintenance of adherens junctions, cell cycle components and members of the EGF pathway. During the screen, I identified six P-element induced lethal mutations which, at the time, were thought to disrupt previously uncharacterised genes. All six have modifying activity comparable to that of ZW3 and DE-Cadherin. In the course of characterising these mutants, I found that two of them disrupted previously characterised genes. One gene encodes a regulatory subunit of protein phosphatase 2A and is called twins/abnormal anaphase resolution. Work by others has shown that this gene is required at the anaphase to telophase transition. The second gene encodes the cell adhesion molecule Fasciclin3. Further molecular and genetic analysis of this gene will be described.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available