Use this URL to cite or link to this record in EThOS: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.599414
Title: Investigating the role of aminopeptidase N in Cry1Ac toxicity using transgenic expression and molecular genetic analysis
Author: Gill, M. B.
Awarding Body: University of Cambridge
Current Institution: University of Cambridge
Date of Award: 2000
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Abstract:
Although fourteen putative receptors for δ-endotoxins have been identified to data from midgut tissue of several lepidopteran subspecies, no evidence exists for their functioning as actual receptors in vivo. Of particular interest to work undertaken in this dissertation was the identification of several putative receptors from the midgut of M. sexta, a lepidopteran subspecies which demonstrates sensitivity to Cry1 toxins. One of these putative receptors, an aminopeptidase N (APN), has been extensively studied in this dissertation in an attempt to evaluate its contribution to the insect toxicity mechanism in vivo. To demonstrate that this protein is the only component required to trigger the Cry1Ac induced cytotoxicity observed in M. sexta, the APN has been expressed within a non-susceptibility host, Drosophila melanogaster. In order to achieve this expression, the Gal4 enhancer trap technique has been employed enabling the directed expression of this lepidopteran APN within the larval stages of the Drosophila in both the midgut and mesodermal tissue. The appearance of toxin susceptibility and tissue specific damage in the otherwise non-susceptible dipteran insect provides strong evidence that this APN is a functional receptor for the Bt specific Cry1Ac toxin in vivo. Additional work covered in this dissertation includes the analysis of the distribution of several putative toxin receptors along the midgut of M. sexta and the identification and cloning of six novel, APN putative receptors from several lepidopteran subspecies.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.599414  DOI: Not available
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