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Title: Comparative genomic analysis as a tool for locating novel functional elements in D. melanogaster
Author: Garrett, M. J.
Awarding Body: University of Cambridge
Current Institution: University of Cambridge
Date of Award: 2010
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The thesis starts with the examination of a dataset representing the sequences of expressed small RNAs in D. melanogaster. After an examination of the quality of the sequences and what parameters are appropriate for its post-processing, it is used to identify novel tRNA genes which are then validated by comparative analysis. The degree of conservation of a set of putative microRNAs identified using a similar technique is then examined and used to determine the probability that the dataset represents genuine microRNAs. Further investigation is carried out into the conservation of a specific spatial arrangement of genes within the genome with the aim of determining whether it is associated with functional relationships. Finally, the conservation of elements of the secondary structure of mRNA molecules is examined in an attempt to identify further genes with a specific subcellular oocyte localisation pattern during development: predictions from this are examined experimentally. Throughout the thesis, comparative analysis is used to identify predictions that appear likely to be functional and worthy of further study. The availability of the genomes of 12 different species of Drosophila allows this to be achieved in a high level of detail, providing insight into a range of functional aspects of the D. melanogaster genome.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available