Use this URL to cite or link to this record in EThOS: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.597436
Title: The potential for [rodent and human] neural precursors to generate oligodendrocytes
Author: Chandran, S.
Awarding Body: University of Cambridge
Current Institution: University of Cambridge
Date of Award: 2000
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Abstract:
The long held belief that the central nervous system (CNS) is incapable of repair has been steadily revised in the last 2 decades. Specifically, the finding of limited remyelination in multiple sclerosis raises the possibility that interventional strategies may be developed to promote and supplement the process. Clearly, an understanding of oligodendrocyte lineage in development is paramount to any novel therapeutic manoeuvres. The experimental work presented here attempts to examine developing rodent and human neural precursor derived cultures with an emphasis on the ability of such cultures to generate oligodendrocytes. The availability of in vitro systems that allow the proliferation and differentiation of precursor cells in culture provides a means of investigating the control of lineage potential and the subsequent myelinating capacity of differentiated oligodendrocytes. The results suggest a finite ability to expand neural precursor cultures derived from the developing rodent CNS, though the ability to generate oligodendrocytes is maintained upon expansion. The developing dorsal spinal cord also appears to contain cells, with the potential to generate oligodendrocytes upon exposure to the mitogens EGF and FGF-2. Comparative studies with expanded cultures derived from the developing human spinal cord, whilst confirming expansion, failed to generate oligodendrocytes. The limited ability of oligodendrocytes to myelinate was confirmed, both in rodent in vitro co-culture and in xenografts of human derived cells transplanted into adult rodent demyelinated lesions. The final chapter considers how these findings build upon existing ideas of oligodendrocyte origin and compares and contrasts the differences between developing rodent and human neural precursors with an emphasis on the oligodendrocyte lineage.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.597436  DOI: Not available
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