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Title: Temperature sensitive mutants of fowl plague virus
Author: Almond, J. W.
Awarding Body: University of Cambridge
Current Institution: University of Cambridge
Date of Award: 1978
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Abstract:
The major part of the dissertation concerns the isolation and genetic characterisation of temperature sensitive mutants of an avian influenza virus, Fowl Plague Virus (FPV). Thirty four mutants of the Rostock strain of FPV have been studied by genetic recombination and shown to fall into six high-frequency recombination groups. The hypothesis that each of these groups corresponds to a single genome segment (single gene) of FPV, led to the work reported in the second part of the dissertation. Each recombination group has been assigned to a particular segment of the FPV genome in the following way. Non-t.s. interstrain recombinants have been constructed from a t.s. Rostock parent and another FPV strain (Dobson) whose RNA segments can be distinguished on the basis of electrophoretic migration in polyacrylamide gels. Determination of the parental origin of each gene in a number of recombinants has led to the identification of the gene carrying the t.s. lesion in the mutant parent. A similar analysis of virus specified polypeptides has also allowed the identification of the defective protein. As a consequence of these analyses the coding function of each segment of the FPV genome has been determined. A separate chapter of the dissertation has been devoted to an investigation of the genetic basis of host range of FPV Dobson. Its relevance to the question of virulence in influenza viruses is discussed. The dissertation concludes with a discussion on several interesting features of certain mutants which may give some indication of their functional defect.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.595484  DOI: Not available
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