Use this URL to cite or link to this record in EThOS: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.592573
Title: In vivo measurement of protein synthesis in human malignant and non-malignant tissues
Author: Heys, Steven D.
Awarding Body: University of Aberdeen
Current Institution: University of Aberdeen
Date of Award: 1991
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Abstract:
The 'flooding dose' technique was applied to the measurement of rats of tissue protein synthesis in normal rectal mucosa, benign colorectal tumour tissue and in rectal mucosa of patients with inflammatory bowel disease. A significant elevation in protein synthesis was observed in these pathological conditions when compared with rectal mucosa obtained from patients undergoing haemorrhoidectomy who had no evidence of other colonic pathology. Protein synthesis in liver, in vivo, was measured in groups of patients with colorectal cancer, inflammatory bowel disease and compared with a control group (patients with benign disease of the gastrointestinal tract). A significant elevation of liver protein synthesis in those patients with inflammatory bowel disease, but not colorectal cancer, was observed when compared with the control group. The acute effects of nutrients on tumour tissue and skeletal muscle protein synthesis were also investigated. Protein synthesis was then measured, using the 'flooding dose' technique, in both tumour tissue and skeletal muscle. A significant elevation of both tumour and skeletal muscle protein synthesis was found in those patients receiving intravenous nutrition compared with the fasted group. Measurement of protein synthesis in a wide range of tissues is possible using the 'flooding dose' technique. Some of these tissues are amenable to percutaneous biopsy or endoscopic biopsy. However, some tissues can only be obtained at the time of surgery and therefore these patients will have been exposed to anaesthetic agents. The effects of anaesthetic agents on tissue protein synthesis, in vivo, are poorly documented. Therefore, a study was undertaken to investigate the effect on tissue protein synthesis of commonly used anaesthetic agents using an animal model.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.592573  DOI: Not available
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