Use this URL to cite or link to this record in EThOS: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.590137
Title: The in vitro fermentation characteristics and prebiotic potential of samples derived from guar gum and potato fibre
Author: Cupit, Kerry
Awarding Body: University of Reading
Current Institution: University of Reading
Date of Award: 2013
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Abstract:
A range of novel and commercially available guar gums and novel potato fibre samples were investigated to see if their fermentation characteristics would make them new potential prebiotic candidates. Samples were examined using in vitro. pH controlled, 24h hatch culture fementations inoculated with human faeces. Fermentation of the guar gum samples mainly stimulated the growth of Bifidobacterium spp. and Bacteroides spp., producing considerable amounts of acetate, propionate and large amounts of gas. A direct relationship between molecular weight (MW) and fermentability was not found but instead two possible optimum molecular weight ranges - MW >500,000 Da and those with much lower MW (-34,000 Da)were suggested. Potato fibre samples produced by pectinase treatment and hemicellulase treatment gave the highest increases in bifidobacteria and lactobacilli, producing considerable amounts of acetate and propionate. Three guar gum samples and One potato fibre sample were investigated further using a three-stage human gut model. The MW of guar gum and its combination were found to affects its fermentibility, bacterial and SCFA profiles, with those containing low MW fractions (34,500 Da) giving higher increases in bifidobacteria and lactobacilli than those containing high MW fractions. Potato fibre treated with pectinase treatment 2 was found to significantly increase bifidobacteria in vessel I (VI) and vessel 2 (V2) and lactobacilli by over 1 log10 in all three vessels. Propionate was also increased significantly in all three vessels. Overall, guar gum and potato fibre derivatives have been shown to exhibit positive fermentation characteristics and prebiotic properties by increasing the beneficial bacteria such as bifidobacteria and lactobacilli and the production of SCFA. This in combination with other health promoting properties and desirable properties to food manufactures make their future use as functional food ingredients encouraging. Further investigation of these samples in human volunteer studies is required to confirm the prebiotic effect and to study potential beneficial properties
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.590137  DOI: Not available
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