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Title: Dissecting the role of presynaptic GABAA recepors in nerve terminal function
Author: Long, Philip
Awarding Body: University of London
Current Institution: University College London (University of London)
Date of Award: 2007
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Fast synaptic inhibition in the mammalian brain is mediated principally by GABAA receptors, a large and diverse family of cr permeable ion channels. Emerging evidence points to the presynaptic localisation of these receptors and their role in regulating intraterminal Ca2+ levels and neurotransmitter release in some areas of the CNS. We used purified nerve terminals (synaptosomes) to establish the presence of functional presynaptic GABAA receptors in the rat neocortex. As a biochemical read-out of presynaptic GABAA receptor activity, we measured Ca2+-dependent changes in the phosphorylation state of synapsin 1. A dose-dependent decrease in synapsin I phosphorylation was detected in response to bicuculline, picrotoxin and GABase, while muscimol and isoguvacine caused a dose- dependent increase in synapsin I phosphorylation. Immunohistochemical analysis revealed this phospho-form of synapsin I to be localised to glutamatergic nerve terminals. In functional studies, GABAA receptor activation by muscimol caused a dose-dependent inhibition of glutamate release, which was abolished by picrotoxin. In order to investigate the mechanism(s) by which presynaptic GABAA receptor activity leads to inhibition of glutamate release, we measured release in the presence of the NKCCl antagonist bumetanide, which abolished the muscimol-induced inhibition of glutamate release. When we subsequently investigated the role ofvoltage-gated Ca2+ channels on muscimol-dependent inhibition of glutamate release, we found that muscimol inhibited glutamate release in the presence of co-conotoxin GVIA, ro-agatoxin IV A and NiCl-. However, the inhibition of release by muscimol was abolished by SNX-482 and nifedipine. Muscimol-dependent decrease in release was also abolished in the presence of W7. Our results indicate that the activation of presynaptic GAB AA receptors in the rat neocortex is coupled to depolarisation of the nerve terminal membrane, leading to inhibition of glutamate release via Ca2+_ dependent Ca2+ channel inhibition linked to L- and R-type VDCCs.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available