Use this URL to cite or link to this record in EThOS: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.584226
Title: Metamorphosis in teleosts : a comparative study between the flatfish Hippoglossus hippoglossus and the round fish Sparus auratus
Author: Campinho, Marco Antonio
Awarding Body: Cardiff University
Current Institution: Cardiff University
Date of Award: 2007
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Abstract:
Metamorphosis is the developmental process by which larvae become adult-like juveniles. In both amphibia and flatfish thyroid hormones (THs) have been shown to drive metamorphosis and probably also influence the larval to juvenile transition in roundfish. Remarkably little is known about the regulation and expression of deiodinase genes that are responsible for TH metabolism or the TH-responsive genes at this developmental stage in teleosts. The objective of this thesis was to characterise the potential role of deiodinases in the metamorphosis of the Atlantic halibut (Hippoglossus hippoglossus), a flatfish and the analogous larval to juvenile transition in a roundfish the gilthead sea bream (Spams auratus). Fast and slow muscle Troponin-T genes were cloned from both species and found to be differentially regulated by THs. Species specific differences were found in TnT genes and their expression profiles during development which probably reflect the significant differences in muscle ontogeny between these teleosts. A halibut epidermal-keratin gene was also isolated and shown to be differentially expressed in skin during metamorphosis and related to molecular, cellular and histological changes that occur at this time. Genes encoding all three deiodinase enzymes (which regulate intracellular TH levels in target tissues) were isolated from both halibut and sea bream and their expression profile was correlated to molecular and cellular changes in muscle during the larval to juvenile transition in both teleosts. The results presented here strongly suggest that despite the significant morphological differences that exist between halibut metamorphosis and sea bream larval to juvenile transition the overall relationships between deiodinases expression is conserved suggesting that THs have a similar role in both these processes.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.584226  DOI: Not available
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