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Title: Molecular analysis on the superfamily of surface antigens from the apicomplexan parasite Eimeria tenella
Author: Ramly, Nur Zazarina
Awarding Body: University of Sheffield
Current Institution: University of Sheffield
Date of Award: 2012
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Abstract:
The work in this thesis has been involved with molecular and structural analysis on the superfamily of surface antigens from the apicomplexan parasite Eimeria ten ella, which causes coccidiosis in chickens. Infection by Eimeria is thought to involve a role for a superfamily of more than 60 cysteine-rich surface antigens (SAGs) in host-parasite interactions. These cysteine-rich SAGs can be divided into two families each with two clusters (A and Bf C and D) within which considerable sequence similarity can be seen. A representative member of the family, SAG 19 (cluster A), has been over-expressed in E. coli, purified and crystallised by the hanging-drop method of vapour diffusion using ammonium sulphate as the precipitant. Crystals of SAG 19 diffract to beyond 1.32 A resolution and belong to space group 14 with unit cell dimensions a = b = 108.1 A, c = 37.42 A with a single subunit in the asymmetric unit.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.577790  DOI: Not available
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