Use this URL to cite or link to this record in EThOS: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.574574
Title: Uterine natural killer cells and related cytokines in reproductive failure
Author: Mariee, Najat Girera
Awarding Body: University of Sheffield
Current Institution: University of Sheffield
Date of Award: 2011
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Abstract:
The thesis begins with a literature review of the physiology 'of implantation and the function of the endometrium with a special emphasis on uterine natural killer (uNK) cells and related cytokines (chapter I). It is followed by an investigation into the reproducibility of uNK cell counts to assess the usefulness of this method as a diagnostic tool (chapter II). Endometrial sections were immunostained for CD56+ uNK cells and analysed by traditional cell counting (TCC) method and image analysis (lA) using Photoshop. The results of TCC method showed that intra-observer and inter-observer agreement on classification of a normal and high uNK cell numbers was good. The results of lA method showed that intra- observer agreement on classification of a normal and high uNK ceJI numbers was also good, while inter-observer agreement was satisfactory. These findings suggest that the measurement of uNK cells in the endometrium has a reasonable degree of precision and may be used for clinical diagnosis. In chapter IV, the results of uNK cell measurements by flow cytometry and immunohistochemistry were compared. The results showed a significant correlation between the two measurements. The cycle-to-cycle variation of uNK measurement was examined in chapter V. The mean difference in uNK cell count between the cycles studied was 5.1 %. Overall, there was a modest cycle-to-cycle variation in uNK cell measurements leading to disagreement of results in 30% of cases. The next section of the thesis (chapter VI) investigated the number of uNK cells in women with RIF. It was found that women with RIF had significantly higher numbers ofuNK cells in the endometrium compared to the control women. In chapter VII, the results of uNK cell measurements were correlated to a number of auto-immune markers including antiphospholipids antibodies (APAs) and thyroid 17 Abstract peroxidase antibodies. The results showed no significant difference in uNK cell numbers between women who had unexplained RM who tested negative or positive for auto-antibodies. Similarly, there was no significant difference in uNK cell numbers between women who had unexplained RIF who tested negative or positive for auto- antibodies. Finally, in chapter VIII, the results of uNK measurements were correlated to IL-15 and LIF expression. It was found that expression of LIF in endometrial glandular epithelium was significantly lower in women with RIF compared to control women. In contrast expression of IL-15 in the stroma was significantly higher in women with RIF compared to control women. There was a significant correlation between uNK cell numbers and stromal expression of IL-15 but there was no correlation between LIF expression in any compartment and uNK cell numbers. The results suggest that IL-15 may play a role in the control ofuNK cell function or proliferation. 18
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.574574  DOI: Not available
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