Use this URL to cite or link to this record in EThOS: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.574540
Title: Use of RNA aptamers to further elucidate the role of FMDV RNA-dependent RNA polymerase (3Dpol) in replication
Author: Forrest, Sophie
Awarding Body: University of Leeds
Current Institution: University of Leeds
Date of Award: 2011
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Abstract:
Replication of poliovirus involves complexes between the viral RNA-dependent-RNA polymerase 3Dpol, its precursor 3CD and the primer Vpg, although the details of replication initiation and elongation are not fully understood. Much less is known about replication complexes of other picornaviruses, especially FMDV. To gain insight into these mechanisms, we carried out in vitro selection to produce RNA aptarners to FMDV 3Dpol. Aptamers are small nucleic acid molecules that can bind targets with affinities and specificities to rival those of antibodies, but offer significant advantages as molecular tools. They are generated by an in vitro selection process termed SELEX and can be modified chemically for use both in vitro and in live cells and to facilitate their detection.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.574540  DOI: Not available
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