Use this URL to cite or link to this record in EThOS: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.571849
Title: Effect of stress and diapause in two Calliphoridae species
Author: Johnson, Bobbie
Awarding Body: University of Birmingham
Current Institution: University of Birmingham
Date of Award: 2013
Availability of Full Text:
Access from EThOS:
Access from Institution:
Abstract:
Cultures of two Dipteran flies (Calliphora vicina (R-D) and C. vomitoria (L.)) were established to answer questions in regards to responses to thermal and desiccation stress, effects of diapause and the mechanisms which underpin diapause. The findings are divided in to two sections. Unequivocal new findings – Calliphora vomitoria were seen to depend on water being present in culture medium for increased survival. Furthermore, C. vomitoria were found to have lower desiccation resistance than C. vicina. Larvae of C. vicina and C. vomitoria showed different cold tolerance strategies, with C. vicina being freeze-avoiding and C. vomitoria ‘partially’ freeze-tolerant. Metabolomics, using \(^1\)H-NMR, revealed that diapause and non-diapause had distinct metabolic profiles. Diapause larvae were seen to reduce energy synthesis from the Krebs cycle and increase glycolysis. Calliphora vicina and C. vomitoria also exhibited different diapause phenotypes; C. vicina entered a maternally regulated facultative diapause as an L3 larvae, Calliphora vomitoria had a less distinct diapause, with maternal conditions having little effect. Speculative new findings - Despite the above differences C. vicina and C. vomitoria were able to produce a viable cross, though field fresh C. vomitoria were not used, as such it cannot be confirmed if this could occur in the wild. Increased temperatures due to climate change may affect both phenology and survival of insects; C. vicina was seen to have a delayed induction to diapause and a reduction in the proportion entering diapause. Diapause conferred increased cold tolerance; therefore those insects that overwinter not in diapause may suffer increased mortality.
Supervisor: Not available Sponsor: Natural Environment Research Council (NERC)
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.571849  DOI: Not available
Keywords: QH Natural history
Share: