Use this URL to cite or link to this record in EThOS: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.570536
Title: Miniaturised opto-fluidic systems for cell manipulation and analysis
Author: Zarowna-Dabrowska, Alicja
Awarding Body: University of Strathclyde
Current Institution: University of Strathclyde
Date of Award: 2011
Availability of Full Text:
Access from EThOS:
Access from Institution:
Abstract:
This PhD research project aimed to develop miniaturised opto-fluidic systems for cell manipulation and analysis which are essential for new medical technologies. These miniaturised systems have distinctive advantages over traditional analysis tools such as faster assays, smaller size, lower cost and much lower sample consumption. In this work, three miniaturised systems were achieved. The first system, miniaturised optoelectronic tweezers (OET), was developed by using a CMOS-controlled GaN micro-light emitting diode (micro-LED) array as an integrated micro-light source. It was demonstrated for the first time that with the spatio-temporal and intensity controllabilities of the emission pattern, the green micro-LEDs are capable of creating reconfigurable virtual electrodes to achieve optoelectronic tweezing. The second system, a miniaturised cell analysis platform, used piezoelectric printing to create micro-patterns of proteins onto a new type of non-adhesive surface, which was then integrated with a microfluidic device. The printed protein pattern has a higher resolution in comparison with previous reported results and remained stable even after extensive washing. Cells seeded on these patterns formed well defined micro-arrays which were successfully used for toxicity studies. The cell micro-arrays were also integrated with a microfluidic device. The third system developed was a miniaturised local cell fluorescence analysis platform. In this system, micro-LEDs with a dedicated thin-film filter and a lens were integrated with a microfluidic network to achieve efficient local fluorescence excitation and detection. By using this system, fluorescence from individual cells has been successfully detected. With their distinctive advantages, the new miniaturised systems developed in this work offer a wide range of clinical and bio-applications.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.570536  DOI: Not available
Share: