Use this URL to cite or link to this record in EThOS: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.570087
Title: Commissioning of an intense broad band source of terahertz radiation and terahertz exposure of biological molecules
Author: Holder, Gareth Michael
Awarding Body: University of Liverpool
Current Institution: University of Liverpool
Date of Award: 2011
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Abstract:
This thesis discusses the commissioning of the most intense broadband source of terahertz (THz) radiation in Europe, at ALICE (Accelerators and Lasers In Combined Experiments), and the first to be equipped with a Tissue Culture Facility (TCF). The commissioning of the THz transport system is discussed in a chronological manner with experimental results compared with theoretical models to improve understanding of the ALICE accelerator and transp€H{'tl1e THz radiation to the TCF. This was first achieved in March 2011. The studies explain some of the complex issues that had to be resolved and the development of a new front end extraction system to maximize the transport efficiency of the beam line. The THz beam line at ALICE has the huge potential to test the long debated and very controversial Frohlich hypothesis concemmg the mechanism of biological organisation. ALICE's time structure results in high peak power, low average power THz radiation. One of the initial studies on cells performed at the ALICE THz beam line is discussed with a number of subsidiary studies performed to clarify the results. A study of THz exposure of Zebra fish embryos provides very interesting results, however at present, it is not possible to state whether the THz radiation has an effect on the growth of Zebra fish embryos due to a number of complications, which are all discussed. The results suggest a possible new hypothesis, where the effects of THz radiation are dependent on whether a biological system enters an arrested state. In this state, the embryo has no ability to fix any damages caused by the THz radiation. The final section of this thesis covers a study of conformational changes of a biological sugar, heparin. Heparin is a structural analogue for Heparan Sulfate which is the most abundant molecule found on a cell surface and plays a crucial role in all reactions and general structure of the extra cellular matrix (ECM). The Reflection Anisotropy Spectroscopy studies suggest that some of the cation forms of heparin dry into a film with an anisotropic direction. The THz radiation results, although not thoroughly understood suggest that THz can distinguish between heparin cation forms due to their differences in conformation. This technique has the long term iv -, potential to monitor conformational change and could contribute to the understanding their reaction with other ECM molecules. v
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.570087  DOI: Not available
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