Use this URL to cite or link to this record in EThOS: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.560224
Title: Proton assisted dissolution of the dental hard tissue enamel as a non-bacterial process
Author: McGeouch-Flaherty, Carrie-Anne
Awarding Body: University of Warwick
Current Institution: University of Warwick
Date of Award: 2011
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Abstract:
The overall aim of this thesis was to examine the kinetics of proton-promoted dissolution of the dental hard tissue enamel as a non-bacterial process and the evaluation of inhibitors with the intent of minimising the dissolution process and effectively protecting the surface. A novel approach was taken, utilising scanning electrochemical microscopy (SECM) to galvanostatically generate controllable and well defined proton fluxes in defined areas of the surface. The resulting etch pits formed on the surface were characterised by optical microscopy and white light interferometry (WLI), which quantitatively determined etch pit dimensions. A theoretical finite element model (FEM) was used to elucidate the kinetics of dissolution based upon the analysis of the shape and dimensions of etch pits produced. A heterogeneous rate constant of dissolution of 0.08 ± 0.04 cm s-1 was attributed to untreated enamel, whereas 2 min treatment with 1000 ppm sodium fluoride (NaF) decreased this rate constant slightly to 0.05 ± 0.03 cm s-1. The impact of fluoride on the rate of proton attack was evident from the formation of shallower broader etch pits. In relation to both acid erosion and caries, the two most relevant acids pertinent to enamel dissolution are citric acid and lactic acid. These acids were investigated by protonating their respective sodium salts in-situ to produce localised weak acid directly under the probe tip. This permitted the surrounding enamel sample to remain largely unaltered giving a true surface for comparison, whilst allowing evaluation of the kinetics in the presence of each weak acid. Etching in the presence of lactic acid, showed a surface controlled process with a rate constant of 0.1 ± 0.03 cm s-1. Etching in the presence of the triprotic citric acid, also yielded a surface controlled process with a rate constant of 0.35 ± 2.6 cm s-1. Calcite was also investigated using SECM, WLI and FEM to validate the use of these techniques. The kinetic data extrapolated was comparable to rate constants found in literature, confirming the validity of these methods. In this case, a novel approach was the use of experimental data to parameterise the finite element model directly. Confocal laser scanning microscopy (CLSM) coupled with SECM was used to visualise proton fluxes from the tip of the UME. This allowed, not only, correlation of the current applied to the probe tip with the pH, but also quantitative data on the spread of protons across a particular surface. Rate constants found for untreated and fluoride-treated enamel were comparable to those found in SECM etching, however, zinc ion treatment proved to result in much greater inhibition of dissolution than fluoride.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.560224  DOI: Not available
Keywords: QD Chemistry ; RK Dentistry
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