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Title: Functional investigation of Trypanosoma brucei microtubule associated proteins and their role in cellular morphogenesis
Author: Towers, Katie
Awarding Body: Lancaster University
Current Institution: Lancaster University
Date of Award: 2010
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The Trypanosoma brucei cytoskeleton is generated by an elaborate array of subpellicular microtubules. This corset of microtubules . requires extensive remodelling during cell growth and division. Microtubule nucleation/outgrowth and coordinated severing/re-establishment of inter-microtubule cross-links is orchestrated by microtubule associated proteins (MAPs). The T. brucei genome encodes a discrete set of trypanosomatid specific MAPs but functional data for most of these proteins is sparse. Through bioinformatic analysis we have identified a novel trypanosomatid-specific protein (GB4L). GB4L has a functional role in trypanosome morphogenesis and microtubule organisation in the procyclic and bloodstream form of the parasite. RNAi ablation of GB4L causes a cytokinetic defect, as does depletion of TCP86 (another novel and trypanosomatid-specific MAP recently identified in the McKean laboratory). Electron microscopy was used to examine both the GB4L and TCP86 RNAi cell lines, demonstrating that the phenotypes observed after GB4L and TCP86 protein depletion are very distinct. However, in both cases protein depletion causes morphological abnormalities at the posterior end of cells. ) ganisation of sub pellicular microtubules was interrogated through localisation of canonical plus tip binding proteins (+ TIPs) EBl and XMAP215. Microtubule plus ends are organised in a highly reproducible pattern throughout the cell cycle. This organisation becomes disrupted when GB4L or TCP86 are depleted, showing that •.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available