Use this URL to cite or link to this record in EThOS: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.554337
Title: Isolation and characterisation of viridans group streptococci from patients with cystic fibrosis
Author: Maeda, Yasunori
Awarding Body: University of Ulster
Current Institution: Ulster University
Date of Award: 2010
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Abstract:
Viridans group streptococci (VGS) are commensal bacteria inhabiting the human oral-nasopharynx, gastrointestinal and urogenital tracts. Recent studies have revealed various aspects of VGS, including possible pathogenesis in patients with cystic fibrosis (CF). This thesis aims to investigate molecular identification techniques for the VGS thus allowing the population structure of VGS to be determined in adult patients with cystic fibrosis, as well as their molecular characterisation in terms of fluoroquinolone and macrolide resistance. The thesis consists of 10 chapters, including a literature review and eight experimental chapters and general discussion. Chapter 1 provides an up-to-date review of the current literature involving the identification and characterisation of VGS organisms. The first experimental theme is the development of a molecular technique for the reliable speciation of members of the VGS into distinct species, as well as an exploration of other novel molecular markers, including the development of a novel PCR assay which uses clustered regulatory interspaced short palindromic repeats (CRlSPRs)-like sequence. Having established methods to identify these organisms, these techniques formed the foundation for a study describing the population structure of VGS in adults patients with CF. Possible pathogenic mechanisms and molecular epidemiology of the resulting VGS populations were examined, as well as the frequency of antibiotic resistance in VGS organisms. Emergence of antibiotic resistance in VGS is major concern as seen in other pathogenic bacteria. Antibiotic resistance in VGS was further characterised in terms of its specific.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.554337  DOI: Not available
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