Use this URL to cite or link to this record in EThOS: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.552079
Title: A study of the behaviour and interactions of the novel FERM protein Willin
Author: Herron, Lissa Rocha
ISNI:       0000 0001 3711 8887
Awarding Body: University of St Andrews
Current Institution: University of St Andrews
Date of Award: 2008
Availability of Full Text:
Access through EThOS:
Access through Institution:
Abstract:
Willin is a novel member of the Four-point-one Ezrin Radixin Moesin (FERM) protein superfamily, containing an N-terminal FERM domain most like the Ezrin-Radixin-Moesin (ERM) family but also the closely related protein Merlin. Willin was initially discovered as a yeast two-hybrid binding partner of neurofascin155, and this interaction has now been confirmed by both co-localisation studies and the use of two different biochemical methods. Like neurofascin155, Willin also localises to detergent resistant membranes, and like the ERM family, it is able to bind to phospholipids. The expression of Willin appears to be toxic as the production of cell-lines stably expressing Willin proved to be not possible and this appears to be because it induces apoptosis in cultured cells. This is a proliferation control function consistent with the suggestion that Willin is the human homologue of the Drosophila tumour suppressor ‘Expanded’. Three antibodies to Willin were also characterised and a novel splice variant, Willin2, subcloned into a GFP-tagged plasmid for comparison with the original form.
Supervisor: Gunn-Moore, Frank J.; Guild, Simon Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.552079  DOI: Not available
Keywords: Willin ; FERM ; Merlin ; Ezrin ; Radixin ; Moesin ; Neurofascin ; L1 ; QP552.C96H4 ; Cytoskeletal proteins ; Gene expression
Share: