Use this URL to cite or link to this record in EThOS: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.545370
Title: Study of gigaseal formation in patch clamping using nanotechnology
Author: Malboubi, Majid
Awarding Body: University of Birmingham
Current Institution: University of Birmingham
Date of Award: 2012
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Abstract:
This PhD project is set out to study the mechanism of gigaseal’ formation and to provide techniques which enable the frequent formation of high resistance seals. Such techniques are highly desirable at both research and industrial levels. The research can be divided into three main sections. In the first section the most important factors in seal formation were identified. Then a group of novel approaches have been developed to alter these factors and achieve better sealing conditions. In the second section the effect of roughness, hydrophilicity and tip size on gigaseal’ formation has been studied. Patch clamp experiments were carried out using conventional and treated pipettes. In the third section of this research, glass micropipettes were characterized using various measurement techniques. The proposed techniques in this thesis not only explain the gigaseal formation in more detail, but also provide techniques to increase both the seal resistance and probability of seal formation. FIB polished pipettes improved the seal resistance significantly and chemically treated pipettes resulted in the formation of seals for more than 80% of trials. Therefore the research aims have been successfully met.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.545370  DOI: Not available
Keywords: TJ Mechanical engineering and machinery ; TS Manufactures
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