Use this URL to cite or link to this record in EThOS: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.529253
Title: The mucus barrier : immune defence against gastrointestinal nematodes
Author: Hasnain, Sumaira
Awarding Body: University of Manchester
Current Institution: University of Manchester
Date of Award: 2010
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Abstract:
Trichuriasis, caused by the intestinal nematode Trichuris, is a disease that affects up to a billion people worldwide. To date, most of our knowledge of this disease comes from the mouse model, Trichuris muris, which has been successfully used to dissect the immune-mediated effector mechanisms that elicit the expulsion of the nematode. Numerous studies have shown a temporal association between intestinal nematode expulsion and goblet cell hyperplasia; however their precise role in response to nematode infection remains elusive. Goblet cells found at mucosal surfaces secrete many constituent components of the mucus barrier, including the gel-forming mucins (Muc2 in the intestine); mucins are large multifunctional glycoproteins that provide the structural framework of the barrier. The studies presented in this thesis demonstrate that the mucosal barrier and in particular its mucin components, changes in response to acute and chronic T.muris infection. In animals resistant to chronic T. muris infection, IL-13-mediated increase in Muc2 production and secretion was observed at the site of infection. Critically, expulsion of the nematode was significantly delayed in the absence of Muc2. Further investigation subsequently showed that Muc5ac, a mucin normally expressed in non-intestinal mucosa was, in fact, expressed in the intestine following nematode infection and was associated with nematode expulsion in the resistant mice. Moreover, mice deficient in Muc5ac were susceptible to chronic infection, despite a strong underlying TH2-type immune response which is essential to eliminate the nematodes, suggesting that Muc5ac acts as a critical effector molecule. Several qualitative changes in the mucins were also noted during resistance: mucins were more highly charged and more sulphated during nematode expulsion. Overall, the changes within the mucus barrier during resistance result in altering the rheological properties of the mucus layer making it less porous and mucins were shown to directly 'damage' the nematodes during nematode expulsion as reflected by a significant reduction in ATP levels. Chronic infection was accompanied by decreased levels of low charged and highly sialylated Muc2. Additionally, we demonstrated that the excretory secretory products released by the nematode consist of serine proteases capable of depolymerising the Muc2 mucin network, which may be part of the nematodes regime to improve its niche and/or aid movement through the mucus layer. Overall, this resulted in a porous mucus layer and a favourable environment for the parasite.Data is presented to show that the intestinal mucus barrier and its constituent mucins are an integral part of the co-ordinated expulsion mechanisms that occur in animals resistant to T.muris infection and we identify a mechanism whereby the nematode exerts its effects on the mucin environment to promote its survival within the host.
Supervisor: Thornton, David ; Grencis, Richard Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.529253  DOI: Not available
Keywords: Mucins ; Nematodes
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