Use this URL to cite or link to this record in EThOS: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.528832
Title: An alginate hydrogel matrix for the localised delivery of a fibroblast/ keratinocyte co-culture to expedite wound healing
Author: Hunt, Nicola Claire
Awarding Body: University of Birmingham
Current Institution: University of Birmingham
Date of Award: 2010
Availability of Full Text:
Access through EThOS:
Access through Institution:
Abstract:
There is a clinical need for effective alternative skin replacements to autografts, allografts and xenografts. In this thesis a bi-layer skin graft was developed by encapsulation of fibroblasts in calcium-alginate hydrogel and culture of keratinocytes on the surface. Initially, the use of 5% and 2% w/v alginate hydrogels were investigated. Both scaffolds maintained fibroblast viability for at least 150 days encapsulation and caused reversible mitotic and catabolic inhibition, as assessed by fluorescent staining, immunochemistry and the thiazolyl blue assay. Sustained expression of angiogenic factors such as vascular endothelial growth factor, interleukin 6 and nerve growth factor were seen by fibroblasts encapsulated in both scaffolds, by reverse transcription polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay. Histological staining demonstrated that following degradation of the scaffolds, fibroblasts secreted ECM to facilitate dermal repair. Comparison of degradation of the scaffolds over time by measuring release of calcium, and changes in rheological properties, morphology and mass, indicated that 5% w/v alginate hydrogel degraded more slowly and was preferable to 2% w/v alginate hydrogel. Fibroblasts encapsulated in 5% w/v alginate hydrogel were shown to express keratinocyte growth factor by RT-PCR, to support keratinocyte proliferation and differentiation, and keratinocytes cultured on the 5% w/v alginate hydrogel surface were seen to form multi-layered epidermal structures by histology, immunostaining and RT-PCR.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.528832  DOI: Not available
Keywords: TP Chemical technology
Share: