Use this URL to cite or link to this record in EThOS: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.525589
Title: Electrochemical biosensors and angiogenesis
Author: Trouillon, Raphael
Awarding Body: Imperial College London
Current Institution: Imperial College London
Date of Award: 2010
Availability of Full Text:
Access from EThOS:
Full text unavailable from EThOS. Please try the link below.
Access from Institution:
Abstract:
Electrochemical methods provide attractive sensing techniques for biology. Electrochemical devices can be easily manufactured, miniaturized and are sometimes the only direct sensing method available. However, stability of these sensors is problematic, as foreign-body type reactions may induce distortions of the signal (biofouling). As a consequence, investigating the interactions with the biological matrix is of paramount importance to achieve reliable sensing. Different types of electrodes (boron doped diamond and different preparations of glassy carbon) and various electrode coatings were tested, in the presence of biological molecules. The results showed that boron doped diamond and fibronectin coated sensors offer good stability, even in the presence of high concentrations of proteins. A generally applicable protocol to assess the quality of electrode materials in biofouling conditions is also presented. Fibronectin has also been found to be a highly biocompatible coating, perfectly suited for cell-based measurements. This fibronectin coating was used on an electrode array to study the pathway leading to angiogenic factor induced nitric oxide release. Vascular endothelial growth factor, a well known angiogenic factor, was initially used and allowed me to setup a reliable and robust protocol for the use of electrode arrays in biology. It was then demonstrated that angiogenin, another angiogenic factor, leads to nitric oxide exocytosis through PI-3 kinase transduction.
Supervisor: O'Hare, Danny ; Parker, Kim Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.525589  DOI: Not available
Share: