Use this URL to cite or link to this record in EThOS: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.511297
Title: Macrocyclic cysteine knot microproteins : total syntheses and biological activities
Author: Thongyoo, Panumart
Awarding Body: Imperial College London
Current Institution: Imperial College London
Date of Award: 2009
Availability of Full Text:
Access from EThOS:
Access from Institution:
Abstract:
Cyclotides are a unique class of head-to-tail cyclic cysteine-knot microproteins that exhibit a wide range of biological activities ranging from insecticidal to anti-HIV. They have a unique structure in which two disulfide bridges form a ring through which a third disulfide bridge is threaded, making cyclotides exceptionally stable against thermal and proteolytic degradation. This characteristic makes them of great interest in drug discovery. MCoTI-I and MCoTI-II, which originally were extracted from Momordica cochenchinesis, are examples of macrocyclic cysteine knot microproteins that display extremely potent trypsin inhibitory activity. In this thesis, two distinct complementary approaches have been developed for the first total synthesis of MCoTI-I and MCoTI-II. In the first, termed thia zip native chemical ligation (NCL), the cyclic backbone and three disulfide bonds are formed in a one pot reaction. In the second, backbone ligation is achieved via a novel immobilised protease-mediated ligation reaction. These methodologies have been further applied to the synthesis of novel analogues of the natural MCoTI-II structure modified at a key residue in the active loop (Lys10) that have altered protease inhibition specificity together with minimizing of MCoTI-II size. These wild-type and re-engineered MCoTI cyclotides were found to be active against not only trypsin-like serine proteases; trypsin, chymotrypsin, leukocyte elastase, HCV NS3/4A, β-tryptase and matriptase, but they are also active against cysteine protease; foot-and-mouth disease virus (FMDV 3Cpro).
Supervisor: Leatherbarrow, Robin ; Tate, Edward Sponsor: European Union for Marie Curie Early Stage Research Training ; Royal Thai Government
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.511297  DOI: Not available
Share: