Use this URL to cite or link to this record in EThOS: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.505476
Title: Development of multidimensional liquid phase protein separations for proteomic analysis
Author: Assiddiq, Bobby Fachrizal
Awarding Body: The University of Sheffield
Current Institution: University of Sheffield
Date of Award: 2009
Availability of Full Text:
Access from EThOS:
Abstract:
This thesis has focussed on the development and application of multidimensional liquid phase protein separations for proteomic analysis. Multidimensional liquid phase chromatography was developed utilising ion exchange chromatography using both cation and anion exchange colums in series in the first dimension. Alternatively, liquid isoelectric focusing protein fractionation was developed first dimension protein separation. Reversed phase liquid chromatography was eloped as a second dimension system, utilising 500 |Lim i.d capillary monoliths. Subsequently, the proteins collected from second dimension were in-solution digested using trypsin and analysed by mass spectrometry. In addition, reversed ase capillary chromatography was coupled directly to ESI-MS to determine intact molecular weights of proteins in a combined top down, bottom up proteomic approach.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.505476  DOI: Not available
Share: