Use this URL to cite or link to this record in EThOS:
Title: Periodicity of genome organization and geneexpression in Streptomyces coelicolor
Author: Marchi, Emmanuele
Awarding Body: University of Surrey
Current Institution: University of Surrey
Date of Award: 2008
Availability of Full Text:
Access from EThOS:
Patterns of transcriptional activity along circular prokaryotic chromosomes such as Escherichia coli and Bacillus subtilis have previously. been characterized through microarray-based analysis of gene expression. However, patterns across linear prokaryotic chromosomes are yet to be investigated. Given the different topological constraints (to circular DNA) imposed upon the linear chromosome the derived transcriptional patterns would be expected to be different. Here we explore transcriptional activity along the linear chromosome of Streptomyces coe/ic%r using expression data from 139 microarrays and genomic features. Representing total chromosomal expression as a spatial series of transcript abundances we observe both short and long range periodicities of transcription along the S. coelicolor chromosome with data derived from different microarray technologies (spotted and ink jet in situ-synthesized). Application of the autocorrelation function confirmed these periodicities as significant both in two and single channel analyses and allowed further analysis of chromosomal properties: Codon adaptation index, GC content, secondary structure and geme length. Another signal processing technique, namely wavelet analysis, has aided the visualization of the periodic signals along the chromosome, identifying 'hot spots' of activity related to specific responses.
Supervisor: Not available Sponsor: Not available
Qualification Name: Not available Qualification Level: Doctoral
EThOS ID:  DOI: Not available