Use this URL to cite or link to this record in EThOS: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.502273
Title: Development of cell culture models to investigate the developmental interactions between breast and adipocyte cell lines
Author: Trice, Gemma
Awarding Body: The University of Strathclyde
Current Institution: University of Strathclyde
Date of Award: 2009
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Abstract:
Breast cancer is the most commonly diagnosed cancer in women and although mortality rates in western countries are improving the incidence continues to rise annually. As obesity reaches epidemic levels globally there is an ever increasing need to develop our understanding of the effects of obesity on malignant processes. This investigation aimed to determine whether factors secreted from adipocytes, which may be altered during obesity, influenced the growth and development of mammary epithelial cells in vitro. The results show limited stimulatory effects from individual growth factors (lGF-1, EGF and HGF) on MCF-lOA cell metabolic activity, apoptosis, cell number and morphology. However, EGF did produce responses suggestive of increased cell migration. Mammosphere 3D structures were successfully achieved using matrigel matrix and these studies demonstrated the ability of TGFpi, TGFP3 and TNFa to disrupt glandular development. In addition a pre-adipocyte cell line 3T3-L1 was successfiilly differentiated into lipid-laden adipocytes. Conditioned medium from these cells in both undifferentiated and differentiated states was used to examine the effects of factors secreted by adipocytes on cellular metabolic activity, apoptosis, and cellular morphology in a normal mouse mammary epithelial cell line, NMuMG. These preliminary results suggested that differentiated adipocytes secrete factors which affect metabolic activity and mammosphere morphology to a greater degree when compared to medium conditioned by undifferentiated adipocytes or unconditioned medium.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.502273  DOI: Not available
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