Use this URL to cite or link to this record in EThOS: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.502262
Title: Isolation and characterization of two mouse mutants with developmental defects
Author: Mitchell, Karen
Awarding Body: The University of Manchester
Current Institution: University of Manchester
Date of Award: 2009
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Abstract:
Congenital abnormalities can be modeled in the mouse using genetic approaches such as phenotype driven mutagenesis screens. Mouse N-Ethyl-N-nitrousea (ENU) mutagenesis screens create point mutations throughout the genome and can be employed to elucidate the function of genes that are conserved between the mouse and human. Region-specific mutagenesis screens can determine the functional content of a defined chromosomal region based on the mutant phenotypes isolated. A region-specific mutagenesis screen directed at mouse chromosome 11 allowed the isolation of the 111Jus27 mutant. Two separate embryonic lethal mutations have been isolated from the lllJus27 mouse line named K27 and embryonic hydrocephalus (EHC). The K27 mouse mutant has defective cardiovascular development causing lethality at mid-gestation. Morphological analysis revealed that pericardial effusion and abnormal cardiac looping are prevalent in K27 mutants. Furthermore, expression analysis indicates that the K27 mutation also causes a disruption in cardiac myogenesis. Meiotic mapping has refined the K27 candidate interval to 5Mb.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.502262  DOI: Not available
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