Use this URL to cite or link to this record in EThOS: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.499626
Title: Microanatomy, structural macromolecules and growth factors expression in the laminar region of the bovine claw
Author: La Manna, Vincenzo
Awarding Body: University of Aberdeen
Current Institution: University of Aberdeen
Date of Award: 2008
Availability of Full Text:
Access from EThOS:
Full text unavailable from EThOS. Please try the link below.
Access from Institution:
Abstract:
The study investigated healthy claw samples from cross-bred heifers aged around 20 months. Histological studies adopted a novel sectioning approach along planes parallel to the wall, identifying dynamics of the keratinisation process, localisation of elastic fibres and anatomical features of cap papillae and horn. Structural molecules were investigated immunohistochemically, including cytokeratins, cytokeratin 14 (CK14) and β-actin. The results showed patterns of suprabasal keratin deposition and loss of signal for CK14 and β-actin markers in differentiating keratinocytes. Localisation of vimentin provided evidence of mesenchymal cells in laminar dermis. Transforming growth factor β1 (TGFβ1) and connective tissue growth factor (CTGF) were investigated immunohistochemically, by in situ hybridisation and RT-PCR. Immunohistochemistry showed signals in the dermis and epidermis, with mRNA presence solely in the dermis and high synthesis rates on comparison with internal control. Mean expression levels were higher in summer than in winter. Collagen I (COL1A2), elastin, estradiol receptor β, cyclin A and B were also investigated by RT-PCR and consistently amplified. Tissue cultures were carried out in order to study TGFβ1, CTGF and COL1A2 expression by RT-PCR in tissue explants incubated in presence of TGFβ1. Results identified a time effect but absence of a concentration effect. Proteomic analysis of fractions extracted from incubated and non-incubated (control) explants showed significant differences for a number of spots, mainly in the keratin region of the electrophoretic gels. In conclusion, the study has provided novel information on molecules of importance in the structure and regulation of epidermal and dermal cells and dermal extracellular matrix in the laminar region of the bovine claw.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.499626  DOI: Not available
Keywords: Lameness in cattle ; Cytokines
Share: