Use this URL to cite or link to this record in EThOS: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.497961
Title: Towards synthetic peptide ligation
Author: Morris, Thomas William
Awarding Body: University of London
Current Institution: University College London (University of London)
Date of Award: 2008
Availability of Full Text:
Access from EThOS:
Full text unavailable from EThOS. Please try the link below.
Access from Institution:
Abstract:
Presented is a novel protocol for the ligation of protected peptide segments that relies on a selective ruthenium-catalyzed cross metathesis reaction. Amino acid A-termini were acryloylated using acryloyl chloride and triethylamine and C-termini were coupled to homoallylamine using standard coupling conditions. The conditions used in both reactions are suited to use on protected peptides. Model studies were performed on a range of single protected amino acids. This demonstrated the applicability of the technique. The synthetic ligation between a protected dipeptide bearing a C-terminal homoallyl amide and a protected 7V-acryloyl tripeptide was achieved. Application to the total chemical synthesis of modified Crambin was then investigated. A site was chosen for ligation and syntheses of the appropriate peptide segments were attempted using solid phases peptide synthesis techniques and an Fmoc protection approach.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.497961  DOI: Not available
Share: