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Title: A study of the interactors of Eps15 homology domain and the role of the EH network in the model system of Caenorhabditis elegans
Author: Tsushima, Hanako
Awarding Body: Open University
Current Institution: Open University
Date of Award: 2008
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Epsl5 homology (EH) domain-containing proteins have been implicated in diverse intracellular signalling pathways, such as endocytosis, actin cytoskeleton organization, nucleo-cytosolic shuttling and mitogenic signalling. However, the extent of the protein-protein interactions mediated by the EH domain at the level of a whole organism has not yet been addressed. This project aims to gain an overview of the EH network in a model system, C. elegans, by identifying interactors of all EH-domain containing proteins present in C. elegans. Five genes encode EH proteins in the C. elegans genome and the isolated EH domains were used to screen a C. elegans cDNA library using the Yeast Two Hybrid system. The validation of the putative interactions was carried out by in vitro pull-down assays. The biological relevance of the interactions was tested genetically using C elegans as a model system. The genetic interactions were monitored using available mutants for four of the five EH encoding genes, in which the genes of the putative interactors were knocked-down by RNA interference.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available