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Title: Antigen presentation by MHC class I and CD1 molecules
Author: Gadola, Stephan D.
Awarding Body: Open University
Current Institution: Open University
Date of Award: 2008
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Antigen presentation is the "sine qua non" of the mammalian adaptive immune stem. The assembly of MHC class 1/peptide complexes in the endoplasmic reticulum (ER) relies on the orchestrated interplay between different chaperonins, which assist MHC class I folding, and the peptide loading complex (PLC). Mutant lymphoblastoid cell lines with defective MHC class I surface expression have greatly helped the functional analysis of the PLC. The TAP transporter associated with antigen processing translocates MHC class I peptide ligands from the cytoso into the ER and is critical for successful MHC class I assembly and maturation. In the first two results chapters of this thesis I will describe the identification, clinical description and molecular and genetic analysis of a new clinical syndrome in a group of patients with dramatically reduced MHC class I surface expression. The disease in these patients could be identified as primary TAP-deficiency. The focus of the third and fourth chapter of this thesis lies on lipid antigen presentation via CDl molecules, which are known to present mycobacterial lipids to T lymphocytes. The first objective of this thesis was to generate recombinant mycobacterial lipid loaded CUl molecules as tools to measure mycobacterial lipid speclfic T cell responses in the TAP-deficient patients.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available