Use this URL to cite or link to this record in EThOS: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.493030
Title: Cren- and euryarchaeal DNA polymerases : interactions with deaminated bases
Author: Gill, Sukhvinder
Awarding Body: Newcastle University
Current Institution: University of Newcastle upon Tyne
Date of Award: 2008
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Abstract:
The family B DNA polymerase from the euryarchaeote, Pyrococcus furiosus (PFU-Pol), has been shown to recognize template strand uracil and stall DNA synthesis four bases ahead, thus preventing the permanent fixation of the mutation. This recognition is due to the presence of a special pocket in the N-terminal domain of the enzyme. This study confirmed the recognition of hypoxanthine, the deaminated product of adenine, by PFU-Pol. Primer extension assays demonstrated that template strand hypoxanthine leads to stalling of DNA synthesis, four bases demonstrated that template strand hypoxanthine leads to stalling of DNA synthesis, four bases in front of the base, exactly the same position as seen for uracil.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.493030  DOI: Not available
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