Use this URL to cite or link to this record in EThOS: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.489971
Title: Regulation of Angiogenesis in Diabetic Vascular Disease
Author: Wang, Binhui
Awarding Body: University of Nottingham
Current Institution: University of Nottingham
Date of Award: 2008
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Abstract:
-Introduction. Angiogenesis promotes neovascularisation in diabetic microvascular complications, e.g retinopathy. Pigment Epithelium Derived Factor (PEDF) is the natural ocular anti-angiogenic protein inversely associated with the development of neovascularisation, but very little is known about either the mechanism underlying its anti-angiogenic property or the regulations of its expression. Aims. The thesis aims to establish the effect of PEDF on key steps of angiogenesis, i.e. endothelial cell proliferation and tubule formation using in vitro proliferation assay and Angiokit assay; and to explore the metabolic and pharmacological regulation of PEDF expression using quantitive PCR and western blotting. Results. PEDF at concentrations of 2 and 10 nM only showed the inhibitory effects on the proliferation of BRCEC (p=0.022 and p=0.018 respectively) and HUVEC (p=0.035 and p=O.017 respectively) at 24 hours. PEDF at 10 nM exerted the inhibitory effect on the number of junctions [145 (19) vs 46(5), p=0.03] and tubules [629(93) vs 311(31), p=O.OOl] and tubule length [11172(2353) vs 4848(748), p=0.001]. On BRCEC and HUVEC, insulin 100 nM reduced the expression of PEDF at mRNA level about 22% (p=0.012) and 33% (p
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.489971  DOI: Not available
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