Use this URL to cite or link to this record in EThOS: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.489319
Title: Ribosome recycling in human mitochondria
Author: Rorbach, Joanna
ISNI:       0000 0001 3534 9833
Awarding Body: Newcastle University
Current Institution: University of Newcastle upon Tyne
Date of Award: 2008
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Abstract:
Despite many years ofresearch, the molecular processes that govern protein synthesis in human mitochondria remain unclear. The main objective in this study is to characterise factors involved in mitochondrial ribosome recycling after translation termination has been completed. Up to now, one protein has been identified bioinformatically as a putative human mitochondrial ribosome recycling factor (mtRRF) but with no supporting experimentation. This study investigates cellular localization and function of this candidate protein. Data presented in this thesis demonstrate that the putative mitochondrial recycling factor is indeed a mitochondrial protein. Further, human !J1tRRF is able to associate with E. coli ribosomes in vitro and with mitoribosomes in vivo. Depletion of mtRRF in human cell lines is lethal, causing a profound mitochondrial dysmorphism, aggregation of mitoribosomes, elevated mitochondrial superoxide production and eventual loss of OXPHOS complexes. Finally, expression of mtRRF in yeast devoid of endogenous· mitochondrial recycling factor suppresses the respiratory phenotype, indicating functional compatibility between these factors. This thesis concludes that mtRRF is an essential protein in mammalian mitochondria directly involved in ribosome recycling and synthesis ofmitochondrial proteins.
Supervisor: Not available Sponsor: Not available
Qualification Name: Newcastle University, 2008 Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.489319  DOI: Not available
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