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Title: Sitka spruce (Picea sitchensis (Bong.) Carr.) propagation technologies : their effects on genetic diversity
Author: Meehan, Eamonn James
Awarding Body: Queen's University Belfast
Current Institution: Queen's University Belfast
Date of Award: 2007
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Abstract:
The aim of this smdy v,'as to assess the genetic diversity of Sitka spruce seedling populations as produced by commercial growers and to detennine what effects the propagation technologies ofrooted cuttings and somatic embryogenesis had on genetic diversity. Seedling populations ,,,ere produced consisting of one hundred seedlings' from each of three families of Washington provenance material and from each of three families of Queen Charlotte Islands provenance. Rooted cutting populations were produced from twelve cuttings from each of fifty randomly selected plants [rom each of the six families. Somatic embryogenesis derived populations (emblings) were produced and fifty populations were provided by Coillte from the three Washington provenance families which represented the maximum number of cell lines produced by a successful commercial grower. Significant phenotypical differences betwe.en zygotic seedlings were found at both family and provenance level after 19 months of grov.1h. All 300 genotypes were able to produce rooted cuttings indicating that no significant loss of genetic diversity in cuttings from two-year-old trees. Seven pairs of microsatellite primers were multiplexed to amplify polymorphic microsatellite DNA loci from needle samples of seedlings and emblings. Capillary electrophoresis of the products yielded data which were analysed according to the Infinite Allele Model and Stepwise Mutation Model. No significant differences were found, using either mutation model, between the genetic diversity values (observed heterozygosity, gene diversity, allelic richness and inbreeding coefficient (Fis)) for the seedling populations and somatic embryogenesis derived populations. A low level of microsatellite variation was found to occur within mature individual trees and within somatic embryogenesis derived populations. The extent of microsatellite variation was found to be dependent on locus and genotype. The propagation technologies of somatic embryogenesis and rooted cuttings do not cause a loss of genetic diversity in the resulting populations.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.486558  DOI: Not available
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