Use this URL to cite or link to this record in EThOS: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.485080
Title: Interfacial Studies of Ternary Phase Separated Biopolymer Solutions
Author: Ward-Passey, Claire Elizabeth
ISNI:       0000 0001 3563 2217
Awarding Body: University of Sheffield
Current Institution: University of Sheffield
Date of Award: 2006
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Abstract:
The physics of phase separated water soluble biopolymer solutions is of great importance both academically and commercially. Of particular importance is the nature of the interface between the two aqueous biopolymer systems. The interface between phase separated water soluble biopolymers has considerable influence over the physical behaviour and properties of mixed biopolymer systems. Knowledge of the nature, and ultimately the ability to control, the aqueous biopolymerlbiopolymer interface is the aim ofthis research. This research examines a protein/polysaccharide biopolymer system: gelatin (a protein that under certain conditions forms a gel) and dextran (a non-gelling polysaccharide). It is known (from small angle light scattering) that the interface between 4.2% aqueous gelatin and dextran solutions is broad:::: 200nm. The phase diagram and the interfacial width of the aqueous gelatin/dextran system have been investigated in this thesis. The phase diagram of this system has been determined using Fourier Transform Infrared (FTIR) spectroscopy. A slight temperature dependence ofxwas found. Spectroscopic and angular ellipsometry have been used in order to try to measure the interfacial width in this system. The aqueous gelatin/dextran system is a liquid/liquid system. A special cell has been designed to enable the two liquids to be confined, but still enable the interface to be studied. A number of test systems (small molecule and macromolecular) have been used in order to test the liquid/liquid experimental cell and the data analysis program. No light was observed to reflect from the interface between aqueous solutions of gelatin and dextran. This is due to the width of the interface being too broad. For the interface between phase separated aqueous solutions (gelatin-rich phase/dextran-rich phase) no light was observed to reflect from the interface due to the width being to broad and the refractive index contrast being too small.
Supervisor: Not available Sponsor: Not available
Qualification Name: University of Sheffield, 2006 Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.485080  DOI: Not available
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