Use this URL to cite or link to this record in EThOS: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.485014
Title: Tissue Engineering of Neuromuscular Junctions, Development of An Alternative to The LD50 Toxicity Test
Author: Huber, Alexander
Awarding Body: University of Nottingham
Current Institution: University of Nottingham
Date of Award: 2007
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Abstract:
Therapeutic Botulinum neurotoxin preparations have found an increasing number of clinical uses in a large variety of neuromuscular disorders and dermatological conditions. The accurate determination of potency in the clinical application of botulinum toxins is critil;al to ensure clinical efficacy and safety, and is currently achieved using a lethal dose (LDso ) assay in mice. Operational constraints and ethical concerns associated with this assay have prompted the development of alternative assay systems that could potentially lead to its replacement. One such tissue-based alternative, the 'Intercostal Neuromuscular Junction (Nl\JJ) Assay', uses nerve:muscle tissue preparations from the rat rib cage. The individual tissue preparations and the experimental protocol were subject to a detailed analysis. The tissue sections used were found to be highly reproducible as determined through the innervation pattern and the localisation of NMJs in situ and their distribution in skeletal muscle fibre types. Furthermore, the efficacy of the assay protocol to successfully deliver the test sample to the cellular target sites ,vas critically assessed using molecular tracer molecules. Sonification during sample aplication resulted in an accelerated way of sample application.
Supervisor: Not available Sponsor: Not available
Qualification Name: University of Nottingham, 2007 Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.485014  DOI: Not available
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