Use this URL to cite or link to this record in EThOS: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.467721
Title: Chemical and biochemical applications of N.M.R.
Author: Orchard, David
Awarding Body: University of Sussex
Current Institution: University of Sussex
Date of Award: 1978
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Abstract:
Measurement of enhancements to the nuclear magnetic spin-lattice relaxation times of solvent water protons were used to probe the complexation of manganese(II) ions with the iron protein of nitrogenase. Enhancement data were used to determine concentrations of bound manganese in solutions containing the metal ions and buffered aqueous protein, and these concentration data treated by the Scatchard plot method to provide binding parameters for the manganese-protein complex. These studies showed four independent, equivalent sites on the protein for binding manganese ions. Competition studies in solutions containing the iron protein and known concentrations of manganese(II) and magnesium(II) ions showed that the four sites available to manganese could alternatively bind magnesium ions.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.467721  DOI: Not available
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