Use this URL to cite or link to this record in EThOS: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.462133
Title: Studies on the phosphorylation of brain microtubular components
Author: Kirazov, Evgeni Penev
Awarding Body: University of London
Current Institution: Royal Holloway, University of London
Date of Award: 1977
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Abstract:
Microtubular protein isolated from brains of adult rats or 1 to 3-day old chicks, previously injected with 32P, contained considerable amounts of serine-bound 32P measured as alkali-labile protein bound 32P. Between 40 and 80% of the acid-stable tubulin-bound 32P was extractable with lipid solvents and was shown to be associated with several major phospholipid classes. Experiments in which tubulin purified by poly-merisation was incubated with [gamma-32P] ATP revealed that tubulin itself acted as a substrate for a microtubule-associated protein kinase under in vitro polymerisation conditions. Further investigations concerning the biochemical properties of brain microtubulin fractions prepared in the presence or absence of glycerol revealed that glycerol brought about a re-distribution of total protein and of phospholipid-and protein-bound 32P. In a final series of experiments, the interactions between myo-inositol and purified brain tubulin were investigated. It was found that myo-inositol binds to tubulin (0.5--1.0 moles of myo-inositol/mole of tubulin dimer) and that myo-inositol affected the stability of in vitro polymerised microtubules. The data presented in this work provide new biochemical evidence for interactions between microtubules and membranes, the functional implications of which are discussed.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.462133  DOI: Not available
Keywords: Biochemistry
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