Use this URL to cite or link to this record in EThOS: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.446579
Title: A physiological, genetic and transcriptomic study of rice-Striga interactions
Author: Kaewchumnong, Krittika
ISNI:       0000 0001 3593 8400
Awarding Body: University of Aberdeen
Current Institution: University of Aberdeen
Date of Award: 2007
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Abstract:
In this thesis, physiological aspects of several hosts and Striga interaction were studied.  Striga­-resistant QTLs were mapped and host gene expression during Striga attachment was analysed in rice. Mapping Striga-resistance QTLs in Bala x Azucena F6 recombinant inbred lines identified a prominent QTL region at an average position 123.2 cM on the Rice Genome Research Program (RGP) map between marker RM246 and RM212 on chromosome 1.  This region contains QTLs for all traits tested with LOD scores ranging from 3.4-10.2 explaining 7.4-36.8% of the phenotypic variation. Database searching between position 121-124.8 cM revealed 47 interesting genes potentially responsible for the effect of Striga on rice. A microarray experiment was conducted to monitor gene expression in Azucena and Bala root upon Striga attachment.  Nine and 25 probe sets were differentially regulated 2 fold or more in Azucena and Bala during Striga attachment, respectively.  Many of these probe sets represent genes involved in stress response, signal transduction, amino acid metabolism and cell death.  There were 2,194 probe sets differentially regulated 2 fold or more between Azucena and Bala seedlings in the control treatment.  Among these probe sets, 11 represent genes in the QTL region for Striga resistance.  These genes are involved in endochitinase, toxin catabolism, signal transduction, starch biosynthesis and response to abscisic acid.  Endochitinase is proposed to be a notable candidate gene for Striga resistance as the gene is induced in both Azucena and Bala upon Striga attachment and is more highly expressed in Azucena compared with Bala in the control treatment.  Results generated from this project can be valuable for the study on host-Striga relationship and for the future breeding experiments to incorporate Striga-resistance genes into elite crop cultivars.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.446579  DOI: Not available
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