Use this URL to cite or link to this record in EThOS: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.431356
Title: Identification and analysis of ferric reductase genes in C. albicans
Author: Mason, Robert Peter
Awarding Body: University of Leicester
Current Institution: University of Leicester
Date of Award: 2006
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Abstract:
In this study a further 15 ferric reductases were identified in the C. albicans genome sequence. The expression of a number of these reductases was examined and CaFRE10 and CaFRE12 shown to be the most highly expressed of those detected. Levels of CaFRE10 transcript increase in response to iron limitation in a manner that is not dependent on the presence of CaAftp. In contrast, CaFRE12 transcript levels increase in response to copper limitation in a CaMaclp dependent manner. The pattern of CaFRE12 expression and sequence comparisons suggest that it is a homologue of the intracellular reductase ScFre7p. Null mutants of CaFRE2, CaFRE5, and CaFRE10 were created during their analysed for phenotypes that were consistent with defects in iron and copper uptake and metabolism. In this study a further 15 ferric reductases were identified in the C. albicans genome sequence. The expression of a number of these reductases was examined and CaFRE10 and CaFRE12 shown to be the most highly expressed of those detected. Levels of CaFRE10 transcript increase in response to iron limitation in a manner that is not dependent on the presence of CaAftp. In contrast, CaFRE12 transcript levels increase in response to copper limitation in a CaMaclp dependent manner. The pattern of CaFRE12 expression and sequence comparisons suggest that it is a homologue of the intracellular reductase ScFre7p. Null mutants of CaFRE2, CaFRE5, and CaFRE10 were created during their analysed for phenotypes that were consistent with defects in iron and copper uptake and metabolism.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.431356  DOI: Not available
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