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Title: Fish cathelicidins : novel antimicrobial peptides identified in rainbow trout Oncorhynchus mykiss and Atlantic salmon Salmo salar
Author: Zhang, Jinyi
Awarding Body: University of Aberdeen
Current Institution: University of Aberdeen
Date of Award: 2006
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This thesis reports a novel antimicrobial peptide gene, termed rtCATH_1, found in rainbow trout Oncorhynchus mykiss.  The predicted 216-residue rtCATH_1 prepropeptide consists of three domains: a 22-residue signal peptide, a 128-residue cathelin-like region containing two identifiable cathelicidin family signatures, and a predicted 66-residue C-terminal cationic antimicrobial peptide.  This predicted mature peptide was unique in possessing features of different known (mammalian) cathelicidin subgroups such as the cysteine bridged family and the specific amino-acid rich family.  A 36-residue peptide corresponding to the core part of rtCATH_1 was chemically synthesised and shown to exhibit potent antimicrobial activity.  Thus rtCATH_1 represents the first teleost cathelicidin. Further to the finding of rtCATH_1 gene, three more cathelicidin genes were found in salmonids; two in Atlantic salmon, named asCATH_1 and asCATH_2, and one in rainbow trout named rtCATH_2.  All the four new salmonid cathelicidin genes share the common characteristics of mammalian cathelicidin genes, such as consisting of four exons, and possessing a highly conserved pre-proregion and four invariant cysteines clustered in the C-terminal region of the cathelin-like domain.  Two 36-residue peptides corresponding to the core part of rtCATH_1 and rtCATH_2 were chemically synthesised and shown to exhibit potent antimicrobial activity.  The differential expression between rtCATH_1 and rtCATH_2 were studied.  rtCATH_2 was expressed constitutively in gill, head kidney, intestine, skin and spleen whilst the expression of rtCATH_1 was inducible in gill, head kidney and spleen after bacterial challenge.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available